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. 2021 Oct 11;11:20131. doi: 10.1038/s41598-021-99696-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of the blocking ability of peptides on proteins of hBMECs. (A) Blocking of the interaction between proteins of hBMECs and rDIII by ELISA. Proteins of hBMECs were coated into microtiter wells. Data present mean of triplicates with ± S.D after subtraction of negative control (rDIII excluded from the assay). A statistically significant difference (P < 0.01, two-tailed P-value, GraphPad Prism v8.4.3.) was calculated by paired t-test compared to the positive control (interaction between rDIII and hBMECs proteins). a: P = 0.0002; b: P = 0.0010; c: P = 0.0058; d: P = 0.0101 (ns); e: P = 0.0015; f: P = 0.0017; g: P = 0.0004; h: P = 0.0021. A – Absorbance. (B) Blocking of the interaction between proteins of hBMECs and rDIII by Western blot. Arrow shows interaction between rDIII and ~ 15 kDa receptor of hBMECs. Positive control – unblocked rDIII incubated hBMECs proteins. Negative control – rDIII was omitted from the protocol. rDIII – recombinant DIII; CP – 7-mer cyclic peptide; LP – 12-mer linear peptide.