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. 2021 Oct 11;11:20111. doi: 10.1038/s41598-021-99521-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of skeletogenesis-related genes in starfish larvae. Expression patterns of vegf (a1–a3), vegfr (b1–b3), ets1/2 (c1–c3), erg (d1–d3), alx1 (e1–e3), ca1 (f1–f3), and clect (g1–g3) were examined at 3 dpf (a1–g1, b1’–d1’), 5 dpf (a2–g2, f2’, g2’), and 7 dpf (a3–g3) by whole-mount in situ hybridization (WMISH). The numbers shown in the lower right corner of each image indicate the number of larvae showing a positive WMISH signal among the larvae examined. Arrowheads indicate the regions or cells expressing each gene. Enlarged images are shown in (b1’–d1’, f2’, g2’). See details in the text. Scale bars: 50 μm.