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. 2021 Oct 11;11:20111. doi: 10.1038/s41598-021-99521-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology and gene expression in TALEN-mediated vegfr knockout starfish larvae. Adult skeleton formation and the expression of alx1, ca1, and clect were examined in control (a–f) and vegfr knockout larvae (g–l). In the panel b, arrowheads indicate adult skeletal rudiments. In panels b and h, the number in the lower right corner shows the number of larvae with skeleton rudiments/the total number of larvae examined. In the WMISH images (c–f, i–l), the number in the lower right corner shows the number of larvae with a positive WMISH signal/the total number of larvae examined. Scale bars: 50 μm.