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. 2021 Oct 11;11:20111. doi: 10.1038/s41598-021-99521-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of phbA and phbB in the starfish. The expression of phbA and phbB was examined by WMISH (a–h) and quantitative PCR (qPCR; i and j). (a–h) The number in the lower right corner shows the number of embryos or larvae showing a positive WMISH signal/the total number examined. Asterisks indicate nonspecific signals for phb genes (see “Methods”). (i, j) The X-axis shows the time after fertilization, and the Y-axis shows the mRNA amounts of phbA (i) and phbB (j) relative to that of EF1α. Data are shown as the mean ± standard deviation. Scale bars: 50 μm.