Fig. 3. Effects of LIPUS irradiation on ameliorating AngII-stimulated myocardial fibrosis in mice. (a) The longitudinal sections of LV tissue stained with hematoxylin and eosin (H&E) and wheat germ agglutinin (WGA) from indicated groups. (b, c) Quantitative analyses of the cardiomyocyte cross-sectional areas stained with H&E (b) and WGA (c). At least 50 cardiomyocytes per group (n>3 per group) were measured randomly using Image-Pro Plus software. (d) The longitudinal sections of LV tissue stained with Masson's method and picrosirius red from indicated groups. (e, f) Quantitative analyses of collagen deposition stained with Masson's method (e) and picrosirius red (f). (g) Protein levels of TGF-β and collagen I in the frozen mice hearts from different groups (n=6 per group) and their densitometric analyses. GAPDH was detected as the loading control. Results are presented as mean±standard error of the mean (SEM), n>3 per group. ns indicates being not significant; ^**P<0.01, ^***P<0.001, ^****P<0.0001. LIPUS, low-intensity pulsed ultrasound; AngII, angiotensin II; LV, left ventricle; TGF-‍β, transforming growth factor-‍β; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.