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The identification of mouse chondrocytes and ALT did not impact the viability of mouse chondrocytes. (A) Phase-contrast micrographs of primary mouse cells after isolation day 1, 3, and 5. (B) Safranin O staining of primary mouse cells. (C) Collagen II immunofluorescence staining of primary mouse cells. Cells were treated by ALT (2.5, 5, and 10 μM) in the presence or absence of IL-1β (10 ng/ml) for 24 h. (D) Chemical structure of ALT. (E) Cell viability was determined by CCK-8 assay.
