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. 2021 Sep 28;12:730312. doi: 10.3389/fphar.2021.730312

FIGURE 6.

FIGURE 6

ALT restrained the phosphorylation of STAT3 induced by IL-1β in mouse chondrocytes. (A) Mouse chondrocytes were exposed to IL-1β (10 ng/ml) at different time points (0, 0.5, 1, 2, 4, and 6 h). Phosphorylation of STAT3 was determined by Western blot. (B) Relative protein expression was qualified by ImageJ software, STAT3 was used as the internal control (n = 3). (C) Cells were treated by ALT (2.5, 5, and 10 μM) with or without IL-1β (10 ng/ml) for 2 h. Phosphorylation of STAT3 was determined by Western blot. (D) Relative protein expression was qualified by ImageJ software, STAT3 was used as the loading control (n = 3). #p < 0.05 vs. control group; *p < 0.05 vs. IL-1β group; **p < 0.01 vs. IL-1β group.