FIGURE 8.

ALT relieved IL-1β-induced NF-κB signaling pathway activation in mouse chondrocytes. Cells were exposed to ALT (10 μM) with or without IL-1β (10 ng/ml) for 30 min. (A) Protein levels of p- IκB, IκB, p-p65, p65 were detected by Western blot. (B) Relative protein expression was qualified by ImageJ software, IκB and p65 were used as the loading control, respectively (n = 3). (C) p65 translocation was observed by Immunofluorescence (scale bar 100 μm). #p < 0.05 vs. control group; *p < 0.05 vs. IL-1β group; **p < 0.01 vs. IL-1β group.