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. 2021 Oct 11;11:20083. doi: 10.1038/s41598-021-99425-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The pcDNA3.1-HPV16-L1 plasmid was successfully constructed and HPV16-L1 was identified to express in BHK cells. (A) The map of pcDNA3.1-HPV16-L1. (B) Identification of pcDNA3.1-HPV16-L1 by restriction enzyme digestion. (M1: DL2 000; lane 12: plasmid digested by HindIII and KpnI; M2: DL15000). (C) SDS PAGE analysis of HPV16-L1 protein expressed in BHK cells (M: protein marker, lane 1: control, lane 2: expressed protein). (D) Western blotting analysis of HPV16-L1 protein expressed in BHK cells (M: protein marker; lane 1: control; lane 2: expressed protein). (E) Immunocytochemistry analysis of HPV16-L1 protein expressed in BHK(× 400). (F) Electron microscropy detection of HPV16-L1 expressed in BHK cells.