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. 2021 Oct 11;11:20083. doi: 10.1038/s41598-021-99425-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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After transfection with pGC-siE6A or pGC-siE7 plasmid, the expression of HPV-E6/E7 protein was decreased in Siha cells. (A) The map of pGC-siRNA. (B) Semi-quantitative RT-PCR analysis of HPV16-E6/E7 mRNA in Siha cells transfected with pGC-siRNA plasmid. (C,D) Western blotting analysis of HPV16-E6 and HPV16-E7 protein in Siha cells transfected with pGC-siRNA plasmid. (E) Transfection efficiency determined by fluorescence microscope. (F,G) Immunofluorescence analysis for HPV16-E6/E7 protein in Siha cells transfected with pGC-siRNA plasmids (× 600).