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. 2021 Oct 11;11:20083. doi: 10.1038/s41598-021-99425-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The pCG-siE6A and pCG-siE7 can promote the apoptosis of Siha cells. (A) Survival ratios for Siha cells transfected with pGC-siRNA plasmids (n = 3). **P < 0.01, vs the control group. (B,C) FCAS analysis for apoptosis rates of Siha cells transfected with pGC-siRNA plasmids. *P < 0.05, **P < 0.01, vs the two control groups. (D) FCAS analysis for cell cycle of Siha transfected with pGC-siRNA plasmids. **P < 0.01, vs the Scramble group. (E) TUNEL analysis for Siha cells transfected with pGC-siRNA plasmids (× 400). (F) Semi-quantitative RT-PCR analysis of mRNA in Siha cells transfected with pGC-siRNA plasmids, **P < 0.01, vs the two control groups. (G,H) Western blotting analysis of related protein in Siha cells transfected with pGC-siRNA plasmids, *P < 0.05, vs the two control groups.