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. 2021 Oct 11;11:20083. doi: 10.1038/s41598-021-99425-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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pCG-siE6 can inhibit the growth of the xenograft tumors in vivo. (A) Growth of Siha xenografts treated with pGC-siE6 (n = 5). Each treatment with the plasmid is shown an arrow. **P < 0.01, vs the two control groups. (B) Semi-quantitative RT-PCR analysis of HPV16-E6/E7 mRNA in Siha xenografts treated with pGC-siE6 plasmids. **P < 0.01, vs the two control groups. (C) HE assay for Siha xenografts treated with pGC-siE6 plasmids (× 400). (D) Fluorescence immunohistochemical analysis of HPV16-E6/E7 protein expression in Siha xenografts treated with plasmids (× 600).