Fig. 3. Effect of Intra-VTA, Intra-CeA and Intra-NAc Administration of LY2817412 on Yohimbine‐Induced Reinstatement of Alcohol Seeking in Male and Female msP rats.

A Schematic representation of the experimental timeline. B–D Self-administration: black circles (male) and white circles (female) represent the mean number of responses during the last 3 days of alcohol self-administration sessions. Self‐administration: black (male) and white (female) circle represents the responses during the last 3 days of alcohol self‐administration sessions. No differences were denoted in the number of active or inactive lever presses during this phase in all brain regions. Extinction: mean number of lever presses during the last 3 days of extinction (EXT). B Male (n = 9) and female (n = 7) msP rats were implanted with bilateral cannulas aimed at the VTA. Compared with EXT, yohimbine elicited a significant reinstatement of responding, both in male and in female rats. Intra-VTA administration of LY2817412 reduced the active lever responses elicited by yohimbine treatment in both sexes. C Male (n = 7) and female (n = 7) msP rats were implanted with bilateral cannulas aimed at the CeA. Compared with EXT, yohimbine elicited a significant reinstatement of responding in female but not in male subjects. Intra-CeA administration of LY2817412 reduced the active lever responses elicited by yohimbine treatment only in female rats. D Male (n = 7) and female (n = 8) msP rats were implanted with bilateral cannulas aimed at the NAc. Compared with EXT, yohimbine elicited a significant reinstatement of responding both in male and female msP rats. Values represent the mean (±SEM). ^##p < 0.01, ^###p < 0.001, difference between EXT and rats treated with yohimbine plus LY2817412 vehicle (0.0); °°p < 0.01, °°°p < 0.001, difference in the reinstatement between male and female msP rats; **p < 0.01, ***p < 0.001, difference between rats treated with yohimbine and LY2817412 vehicle (0.0) and rats treated with different doses of the antagonist.