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. Author manuscript; available in PMC: 2021 Oct 12.
Published in final edited form as: Cell Rep. 2021 Sep 21;36(12):109735. doi: 10.1016/j.celrep.2021.109735

Figure 4. Effect of an additional NF-κB site on HIV-1 sensitivity to human PYHIN proteins.

Figure 4.

(A) Mutations introduced into the LTRs of HIV-1 CH058, ZM247, and CH185 IMCs.

(B and C) HEK293T cells were transfected with either an empty vector or an expression plasmid for human nuclear PYHIN proteins (1 μg) and the indicated proviral constructs harboring the indicated mutations in both LTRs. Infectious virus yield was determined by TZM-bl cells infection assay. Shown are absolute infectious virus yields (B) and the levels relative to those obtained in the presence of the empty control constructs (C). Data represent the mean from three independent experiments, with each measured in triplicates ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.