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. 2021 Sep 28;9:731028. doi: 10.3389/fcell.2021.731028

FIGURE 10.

FIGURE 10

Effects of a NOS inhibitor (L-NMMA) and a NO donor, sodium nitroprusside (SNP) on TEER and tight junction protein ZO-1 of in vitro BBB formed by bEnd3 monolayer. (A) Normalized TEER, (B) confocal images of ZO-1 of in vitro BBB, and (C) comparison of the intensity profiles of ZO-1 along a ∼3 μm line perpendicular to the EC junctions (white lines in the confocal images) under control and after treatment of 10 min 1 mA/cm2 DCS (DCS), or treatment of 60 min 1 mM L-NMMA, or pretreatment of 60 min 1 mM L-NMMA and treatment of 10 min 1 mA/cm2 DCS (L-NMMA DCS), or treatment of 30 min 300 μM SNP (SNP). n = 6 samples for each case in (A) and n = 3 samples for each case in (C). *p < 0.05, compared with the control, @p < 0.05, compared with DCS, #p < 0.05, comparing L-NMMA DCS and SNP with L-NMMA, %p < 0.05, comparing SNP with L-NMMA DCS.