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. 2021 Sep 28;9:731028. doi: 10.3389/fcell.2021.731028

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Copyright © 2021 Xia, Li, Khalid, Bikson and Fu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of DCS on TEER and tight junction protein ZO-1 of in vitro BBB formed by bEnd3 monolayer. (A) Normalized TEER, (B) confocal images of ZO-1 of in vitro BBB, and (C) comparison of the intensity profiles of ZO-1 along a ∼3 μm line perpendicular to the EC junctions (white lines in the confocal images) under control and after 10 min treatment with 1 mA/cm² DCS. The peak intensity of ZO-1 from the control was used for the normalization. n = 120 profiles for junctions between 30 ECs were averaged for each plot. ^∗p < 0.05.