FIGURE 4.

Tumor cell-derived exosomal PTPRO inhibited cancer cell invasion and migration. (A) Schematic illustration of the in vitro transwell co-culture system. (B) Cell migration and invasion assays were used to study the migration and invasion ability of ZR-75-1 cells. THP-1 cells prestimulated with PMA were treated with ZR-75-1-vector-exo or ZR-75-1-PTPRO-exo and ZR-75-1-shCtrl-exo or ZR-75-1-shPTPRO-exo at a concentration of 25 μg/mL or PBS; then, the upper chamber was obtained and incubated with ZR-75-1 cells for 24 h. Original magnification: 200×. Error bars, SEM. *P < 0.05; **P < 0.01; ***P < 0.001 by a one-way ANOVA with post hoc intergroup comparisons.