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. 2021 Sep 17;433(19):167174. doi: 10.1016/j.jmb.2021.167174

Figure 5.

Figure 5

Analysis of SAXS data for AXH-C, 14-3-3 and their complex. (A) schematic structure of the Ataxin-1 protein. The full-length Ataxin-1 protein has 816 amino acids, with a central AXH domain. In this study, a shorter version of the protein including the AXH domain and C terminus was used. It is referred to as AXH-C. The 14-3-3 binding site created upon phosphorylation of S776 (pS776) is marked. (B) Distance distribution functions for 14-3-3, AXH-C and their complex are shown in green, blue and purple respectively. (C) Dimensionless Kratky plots, with same colour coding as in panel B. The grey lines mark the characteristic maximum for globular proteins of 1.107 at sRg = √3. (D) Ensemble model of AXH-C generated by EOM. The AXH dimer is coloured in shades of blue. Various conformations of the disordered C-termini on each monomer are shown as wire model. (E) Crysol fit of the AXH-C ensemble model (black line) to the experimental AXH-C scattering data (blue dots).