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. 2021 Sep 28;12:688488. doi: 10.3389/fgene.2021.688488

FIGURE 8.

FIGURE 8

Expression analysis of genes involved in the polysialylation process. (A) Relative mRNA level of genes involved in the (poly)sialylation process in the hippocampus of St3gal3 mice (HET: n = 21, WT: n = 13). A significant genotype effect on Cadm1 levels was observed (genotype: F1,43 = 4.124, p = 0.0485). We detected a genotype main effect (genotype: F1,43 = 5.624, p = 0.0223) as well as a significant genotype × sex interaction (F1,43 = 4.736, p = 0.0351) on the levels of St8sia2 expression; post hoc analysis revealed an upregulation in HET male mice compared to WT male mice (post hoc p = 0.0083) and HET females (post hoc p = 0.0481). A genotype effect was also observed for St8sia4 (genotype: F1,43 = 7.538, p = 0.0088). (B) Protein levels of the adhesion molecules NCAM (isoforms NCAM-180, NCAM-140, and NCAM-120) and SynCAM 1 in the hippocampus of St3gal3 male mice (HET: n = 7, WT: n = 8). Representative blots are shown in each case. No differences were observed for any of the analyzed proteins. (C) Relative mRNA level of genes involved in the polysialylation process in the PFC of St3gal3 mice (HET: n = 19, WT: n = 14). A significant sex effect was observed in Cadm1 (sex: F1,42 = 5.495, p = 0.0239). Trends toward sex and genotype effects were found for Ncam1 (sex: F1,42 = 3.105, p = 0.0853; genotype: F1,42 = 2.981, p = 0.0916). A significant sex effect on St8sia4 expression was also detected (F1,42 = 5.558, p = 0.0231). (D) Protein levels of the adhesion molecules NCAM (isoforms NCAM-180, NCAM-140, and NCAM-120) and SynCAM 1 in the hippocampus of St3gal3 male mice (HET: n = 7, WT: n = 8). Representative blots are shown in each case. No differences were observed for any of the analyzed proteins. qPCR data were analyzed by two-way ANOVA followed by Sidak post hoc test (when necessary) and presented as mean ± SEM. Western blot data were analyzed by Student’s t-test with Welch’s correction and presented as violin plots with median + quartiles. #p < 0.1, *p < 0.05, **p < 0.01. For simplicity, only genotype effects are indicated in the figures.