FIGURE 6.

Adiponectin (APN) could improve Aβ31‐35‐induced abnormal Bmal1 mRNA/protein expression by inhibiting the activity of GSK3β. (A) Real‐time PCR was used to measure Bmal1 mRNA expression in HT22 cells of the control group, Aβ31‐35 group, and LiCl +Aβ31‐35 group at different time points. (B, C) mRNA levels of Bmal1 at CT12 and CT20 in each group. (D, E) Western blotting analysis showing the protein expression of BMAL1 at CT20. (F) The protein expression of PGSK3β^S9 and GSK3β in the control group, Aβ31‐35 group, APN +Aβ31‐35 group and APN alone group was detected by western blotting. (G) Quantitative analysis of the PGSK3β^S9/GSK3β ratio in each group. Data are expressed as the mean ± SEM (n = 6 per group). *p <0.05 compared to the control group; ^# p < 0.05 compared to the Aβ31‐35 group