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. 2021 Sep 12;25(20):9586–9596. doi: 10.1111/jcmm.16899

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© 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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SeV‐Myod1 and 38℃ treatment converted multiple induced pluripotent stem cells (iPSCs) into skeletal muscle (SkM) cells. (A) Immunostaining of SkM cells originating from three different iPSCs were immunostained by myosin heavy chain (MHC) antibody (green), rBC2LCN‐635 dye (magenta) and nucleus‐stained with DAPI (grey). Scale bars = 100 μm. (B) Positivities of rBC2LCN were calculated. Independent experiment, n = 3, mean ± S.D. (C) Positivities of MHC were calculated. Independent experiment, n = 3, mean ± S.D