FIGURE 5.

Knockdown of lncRNA JPX suppressed the proliferation and metastasis of OS cells. (A) LncRNA JPX was knockdown in OS cells by specific shRNA (shRNA JPX). The transfection efficiency was examined with real‐time qPCR analysis. (B) MTT assay was carried out to determine the cell viability in OS cells transfected with shRNA NC or shRNA JPX. (C) Colony formation assay was performed to test the proliferative ability of OS cells after knockdown of lncRNA JPX. (D) Wound healing assay was then utilized to assess the migration of OS cells after shRNA JPX transfection. (E) Invasion ability was examined in OS cells in shRNA JPX group by using transwell assay. (F) Attachment and detachment assays were conducted to detect the metastasis ability of OS cells transfected with shRNA JPX for 48 h. Scale bar, 250 μm. Significant difference relative to control group was presented as *p < 0.05; **p < 0.01 and ***p < 0.001