FIGURE 7.

Wnt/β‐catenin signalling pathway was involved in lncRNA JPX mediated tumour progression in OS cells. (A) The protein levels of Wnt/β‐catenin signalling target genes were measured by western blot in MG63 cells transfected with lncRNA NC or lncRNA JPX. (B) The protein expression of the core factors of Wnt/β‐catenin signalling pathway was examined by western blot assay in MG63 cells treated with Wiki4. (C) The proliferative ability of MG63 cells treated with lncRNA JPX or lncRNA JPX + Wiki4 was examined with colony formation assay. (D) The invasion increased by lncRNA JPX was decreased in the presence of Wiki4. (E) The migratory ability of MG63 cells treated with lncRNA JPX or lncRNA JPX + Wiki4 was detected by wound healing assay (F‐H) The roles of Wnt/β‐catenin signalling in the inhibition of lncRNA JPX by melatonin. Scale bar = 250 μm. Significant difference relative to control group was presented as *p < 0.05; **p < 0.01 and ***p < 0.001