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Schematic structure of THF, 5-F-THF, and 5-F-THF-Dayne probe. THF consists of three moieties: pteridine, PABA, and glutamate. The N5 and N10 positions in THF are marked in red. In the 5-F-THF-Dayne probe, a “diazirine-alkyne” (“Dayne”) photoaffinity tag was coupled to the γ-carboxyl group. The diazo part “N=N” is indicated in blue and the alkyne moiety in red. For detailed synthesis procedure see “Methods” section. HOBt, hydroxyl-benzotriazole; DIPEA, N,N-diisopropyl-ethylamine.
