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. 2021 Sep 28;12:727585. doi: 10.3389/fphys.2021.727585

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Copyright © 2021 Brown, Davis, Fogarty and Sieck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Representative photomicrographs of mitochondria labeled using MitoTracker Green in DIAm fibers from 6-month and 24-month Fischer 344 rats in alternate serial sections (same fibers used for MyHC fiber type classification and SDH_max measurements) cut at 10 μm thickness. MitoTracker Green fluorescence was visualized using an Olympus FV2000 laser scanning confocal microscope. (B) Fluorescence intensity was thresholded to produce binary images of the MitoTracker Green labeled mitochondria.