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. 2021 Sep 28;12:727585. doi: 10.3389/fphys.2021.727585

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Copyright © 2021 Brown, Davis, Fogarty and Sieck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Representative images of the accumulation of NBT_dfz (SDH reaction indicator) in DIAm fibers from 6- and 24-month Fischer 344 rats at 0, 5, and 10 min during the progression of the SDH reaction. (B) Measurements of mean fiber OD [indicating [NBT_dfz] were obtained every 15 s during the SDH reaction for type I (circles), IIa (squares), and IIx/IIb (triangles) DIAm fibers from both 6-month (purple) and 24-month (green) male and female rats]. The SDH reaction was found to be highly linear (R² = 0.99) during this period. From the slope of accumulation of [NBTdfz] (dOD/dt), the maximum velocity of the SDH reaction (SDH_max) was determined for each fiber. (C) SDH_max normalized to fiber volume was higher in type I and IIa DIAm fibers compared to type IIx/IIb fibers. SDH_max normalized to fiber volume was not different in type I and IIa DIAm fibers between 6-month and 24-month rats. SDH_max normalized to fiber volume in type IIx/IIb fibers was lower than that in type IIx/IIb DIAm fibers in 24-month compared with 6-month rats (two-way ANOVA, *P < 0.05). (D) SDH_max normalized to mitochondrial volume per fiber was higher in type I and IIa DIAm fibers compared to type IIx/IIb fibers. SDH_max normalized to mitochondrial volume per fiber was not different in type I and IIa DIAm fibers between 6-month and 24-month rats. SDH_max normalized to mitochondrial volume in type IIx/IIb DIAm fibers was lower in 24-month compared with 6-month rats (two-way ANOVA, *P < 0.05). # indicated difference between type IIx/IIb fibers and type I and IIa fibers of all ages.