FIG. 1.
Expression and purification of CBFα2. (A) Coomassie blue-stained sodium dodecyl sulfate-polyacrylamide gel displaying fractions from each step of the purification for CBFα2(451) and two truncated derivatives, CBFα2(1-331) and CBFα2(1-214). Lanes: M, molecular weight markers; NE, unfractionated nuclear extract; Ni-NTA, eluate from the Ni-NTA column; αFLAG, eluate from the anti-FLAG monoclonal antibody column. Arrows indicate expected position of the CBFα2 bands. (B) Activities of CBFα2 proteins quantified by DNA titration in an EMSA. Concentrations (molar) of protein-DNA complex [PD] versus total input DNA [Dt] are plotted.