FIG. 5.
Sequences in CBFα2 required for cooperative DNA binding with Ets-1. Equilibrium DNA binding studies were performed by EMSA with truncated CBFα2 proteins in the absence (open circles) or presence (closed circles) of Ets-1 (A to D). Equilibrium DNA binding curves display [PD]/[Dt] as the mean (±standard error) of at least two independent experiments. (E) Summary of equilibrium dissociation constants derived from binding curves in panels A to D. KD values and standard error were obtained from the curve fit of means as described in Materials and Methods.