Table 1.

The features of primers used in this study.

Gene	Primer sequence	Product size	Annealing temp. °C
FTO	Forward outer primer: AGTTCCAGTCATTTTTGACAGC Reverse outer primer: AGCCTCTCTACCATCTTATGTC Forward inner primer: CCTTGCGACTGCTGTGAATATA Reverse inner primer: GAGACTATCCAAGTGCATCTCA	Control fragment: 429bp T allele: 278bp A allele: 194bp	59.5