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. 2021 Sep 28;15:725573. doi: 10.3389/fncel.2021.725573

FIGURE 2.

FIGURE 2

Exosomes educate macrophages to become M2-like macrophages. (A) Analysis of cellular exosome uptake by macrophages. PKH26: red, DAPI: blue, F4/80: green. Scale bar: 20 μm. (B) Macrophage mRNA was analyzed for the indicated M1 or M2 marker genes by RT-PCR (iNOS: t = 17.45, p < 0.01; TNF-α: t = 14.19, p < 0.01; CCR7: t = 21.55, p < 0.01; Argnase-1: t = 7.714, p < 0.01; CD206: t = 10.61, p < 0.01; IL-10: t = 8.737, p < 0.01; n = 3 biological replicates for each group. (C) Changes in surface antigen expression in educated macrophages was analyzed by flow cytometry. (D) Quantitation of percentage of CD86+/CD11b+F4/80+ of (C) (t = 19.39, p < 0.01, n = 3 biological replicates for each group). (E) Quantitation of percentage of CD206+/CD11b+F4/80+ of (C) (t = 80.15, p < 0.01, n = 3 biological replicates for each group). The data are presented as the means ± SD, *p < 0.05, **p < 0.01, NS = Not significant.