FIGURE 3.
Suppression assay of regulatory T cells [regulatory T cells (Tregs)]. (a) Representative histograms showing the proliferation of conventional T cells (con‐T cells) with and without Tregs. (b) Comparisons of proliferative frequency of con‐T cells without Tregs (n = 9) and with Tregs from either acute adult‐onset Still’s disease (AOSD) patients (n = 8) or healthy controls (HC; n = 9). (c) Differences in the proliferative frequency of con‐T cells in the presence of Tregs from five patients collected in the acute and remission phases (n = 5). CFSE = carboxyfluorescein diacetate succinimidyl ester; HC‐Tregs = Tregs from HC; acute AOSD‐Tregs = Tregs from patients with acute AOSD; a‐CD3/CD28 = anti‐CD3/CD28 microbeads; NS = not significant. The Kruskal–Wallis and Steel–Dwass tests were used for comparisons of proliferative frequency of con‐T cells between three independent groups. Wilcoxon’s signed‐rank test was used for comparisons between acute and remission phases in five AOSD patients. *p < 0.05, **p < 0.005