FIGURE 3.

Endothelial cells (ECs) from young donors’ regulated T cells to produce fewer proinflammatory cytokines. Schematic diagram of the study design of the co‐culture of ECs and CD3⁺ T cells and the measurement of effector T subsets after 3 days of co‐culture (a). The frequencies of bone marrow (BM) T helper type 1 (Th1) [CD3⁺CD8⁻interferon (IFN)‐γ⁺] (b), Th2 [CD3⁺CD8⁻interleukin (IL)‐4⁺] (c), T cytotoxic cell 1 (Tc1) (CD3⁺CD8⁺IFN‐γ⁺) (e), Tc2 (CD3⁺CD8⁺IL‐4⁺) (f), Th17 (CD3⁺CD8⁻IL‐17⁺) (i) and regulatory T cells (T_regs) [CD3⁺CD8⁻CD25⁺forkhead box protein 3 (FoxP3⁺)] (j) in young and elderly donors. The ratios of BM Th1/Th2 (d), Tc1/Tc2 (g), CD4⁺/CD8⁺ T cells (h) in young and elderly donors