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. 2021 Sep 28;12:713513. doi: 10.3389/fmicb.2021.713513

TABLE 3.

Alpha diversity estimation of the 16S rRNA gene libraries by sequencing on an Ion S5TM XL platform in bacon.

Sample name Total tags OUTs Shannon Simpson Chao1 ACE Goods coverage
Day 0 CK.0d 71493 ± 14839 312 ± 10 5.878 ± 0.385 0.964 ± 0.011 324.383 ± 7.477 322.247 ± 8.397 0.999
CK.7d 68496 ± 13865 321 ± 15 5.469 ± 0.123 0.933 ± 0.016 328.667 ± 27.319 324.478 ± 20.440 0.999
P2.7d 64053 ± 15249 239 ± 30 4.646 ± 0.916 0.885 ± 0.073 257.667 ± 40.819 258.817 ± 39.047 0.998
P6.7d 69774 ± 11907 210 ± 61 3.774 ± 1.309 0.759 ± 0.186 237.105 ± 62.780 238.501 ± 60.792 0.998
Day 7 P9.7d 80138 ± 43 204 ± 9 3.893 ± 0.520 0.785 ± 0.094 237.285 ± 3.592 233.512 ± 9.352 0.998
P16.7d 77670 ± 4315 269 ± 51 5.516 ± 0.300 0.951 ± 0.011 284.552 ± 55.676 286.245 ± 51.688 0.998
P20.7d 60857 ± 4449 264 ± 1 5.591 ± 0.301 0.957 ± 0.014 286.079 ± 18.371 287.616 ± 13.541 0.998
Pm.7d 62284 ± 11974 223 ± 18 4.679 ± 1.139 0.867 ± 0.118 244.855 ± 15.973 242.537 ± 16.279 0.998
Day 15 CK.15d 79773 ± 685 301 ± 14 5.468 ± 0.527 0.939 ± 0.039 315.205 ± 12.794 310.881 ± 13.723 0.999
P2.15d 61970 ± 13686 165 ± 137 3.563 ± 1.482 0.804 ± 0.110 210.937 ± 131.344 227.950 ± 114.921 0.998
P6.15d 82203 ± 3496 107 ± 100 2.789 ± 1.574 0.704 ± 0.163 127.588 ± 103.329 130.114 ± 103.902 0.999
P9.15d 80076 ± 65 46 ± 10 1.485 ± 0.933 0.430 ± 0.294 66.277 ± 8.910 70.344 ± 18.673 0.999
P16.15d 80117 ± 104 114 ± 57 3.209 ± 0.526 0.811 ± 0.044 145.363 ± 59.737 151.362 ± 56.029 0.998
P20.15d 80111 ± 59 205 ± 104 4.100 ± 1.870 0.869 ± 0.092 243.200 ± 76.977 249.795 ± 75.928 0.998
Pm.15d 61511 ± 16295 63 ± 30 3.213 ± 0.226 0.857 ± 0.041 84.118 ± 41.677 91.340 ± 46.863 0.999
Day 22 CK.22d 73183 ± 12127 293 ± 17 5.616 ± 0.362 0.945 ± 0.027 293.666 ± 17.897 293.666 ± 17.897 1.000
P2.22d 79485 ± 1554 47 ± 6 2.653 ± 0.347 0.754 ± 0.076 57.783 ± 11.145 66.961 ± 14.723 0.999
P6.22d 78300 ± 3148 57 ± 18 2.786 ± 0.071 0.785 ± 0.009 71.444 ± 20.205 74.739 ± 12.082 0.999
P9.22d 80132 ± 68 50 ± 5 2.452 ± 0.052 0.726 ± 0.022 73.174 ± 7.476 81.311 ± 13.151 0.999
P16.22d 80163 ± 59 46 ± 3 2.778 ± 0.224 0.787 ± 0.036 68.714 ± 5.765 84.755 ± 14.827 0.999
P20.22d 62869 ± 6208 99 ± 77 3.229 ± 0.911 0.840 ± 0.057 122.927 ± 85.611 127.332 ± 88.602 0.998
Pm.22d 62525 ± 15307 40 ± 12 3.072 ± 0.443 0.834 ± 0.079 47.952 ± 14.375 53.788 ± 15.813 0.999
Day 30 CK.30d 72405 ± 13556 345 ± 20 5.902 ± 0.522 0.960 ± 0.024 363.222 ± 11.926 355.820 ± 10.079 0.999
P2.30d 69744 ± 9827 39 ± 5 2.643 ± 0.231 0.756 ± 0.046 58.660 ± 5.395 74.742 ± 22.268 0.999
P6.30d 74137 ± 5241 65 ± 12 2.859 ± 0.232 0.789 ± 0.040 92.825 ± 28.131 100.961 ± 35.978 0.999
P9.30d 80127 ± 39 41 ± 5 2.598 ± 0.097 0.753 ± 0.032 60.322 ± 9.067 71.335 ± 17.811 0.999
P16.30d 70178 ± 14083 47 ± 5 2.852 ± 0.034 0.812 ± 0.007 58.403 ± 2.984 65.936 ± 5.569 0.999
P20.30d 58211 ± 6360 52 ± 27 2.739 ± 2.516 0.805 ± 0.013 78.322 ± 43.259 76.269 ± 32.442 0.999
Pm.30d 60813 ± 16677 40 ± 14 3.183 ± 0.175 0.858 ± 0.025 54.166 ± 21.391 61.578 ± 27.871 0.999
Day 45 CK.45d 80115 ± 32 328 ± 6 5.711 ± 0.296 0.953 ± 0.013 346.329 ± 17.584 338.845 ± 9.457 0.999
P2.45d 72141 ± 2356 42 ± 2 2.933 ± 0.112 0.810 ± 0.019 57.400 ± 12.770 63.965 ± 15.422 0.999
P6.45d 76042 ± 7160 51 ± 2 2.883 ± 0.079 0.805 ± 0.014 76.375 ± 5.188 83.236 ± 6.265 0.999
P9.45d 80108 ± 42.158 52 ± 6 2.145 ± 1.109 0.599 ± 0.329 91.277 ± 48.990 91.281 ± 33.496 0.999
P16.45d 59889 ± 6463 66 ± 10 2.915 ± 0.137 0.819 ± 0.014 83.504 ± 10.886 87.552 ± 15.329 0.999
P20.45d 64865 ± 7519 47 ± 11 2.548 ± 0.054 0.788 ± 0.009 65.441 ± 8.010 77.858 ± 13.622 0.999
Pm.45d 60880 ± 16652 56 ± 4 3.309 ± 0.148 0.873 ± 0.018 95.923 ± 22.901 98.431 ± 13.871 0.999

CK, control samples; P2, Staphylococcus xylosus; P6, Leuconostoc mesenteroides; P9, Carnobacterium maltaromaticum; P16, Leuconostoc gelidum; P20, Serratia liquefaciens; Pm, a mixture of these five strains.