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. 2021 Sep;9(17):1374. doi: 10.21037/atm-21-2013

Figure 2.

Figure 2

MiR-552-3p promotes GBC stem cells expansion. (A) The representative images of spheres formed by miR-552-3p knockdown and miR-552-3p control GBC cells (scale bar =100 μm); (B) cloning ability of miR-552-3p knockdown and control GBC cells (dyeing with crystal violet); (C) the percentage of CD44+ in miR-552-3p knockdown and control GBC cells was analyzed by flow cytometry; (D) the proportion of CSC was determined by in vitro limited dilution assay; (E) the expression of stem cell related transcription factors and CSCs markers in miR-552-3p knockdown and control GBC cells were detected by real-time PCR. *, P<0.05. GBC, gallbladder cancer; CSCs, cancer stem cells; T-IC, tumor-initiating cell.