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. 2021 Sep;9(17):1374. doi: 10.21037/atm-21-2013

Figure 3.

Figure 3

MiR-552-3p acts as a key regulator for GBC malignant phenotypes in vitro and in vivo. (A) The proliferation of miR-552-3p knockdown or overexpression in GBC cells was measured by CCK-8 assay; (B) the migratory ability of miR-552-3p knockdown or overexpression in GBC cells was measured by wound-healing assay (scale bar =500 μm); (C) cell migration and invasion were evaluated in GBC cells with miR-552-3p knockdown or overexpression (dyeing with crystal violet; scale bar =100 μm); (D) the GBC-SD cells stably expressing miR-552-3p sponge or sponge NC (Lv-miR-NC) were subcutaneously injected into nude mice (n=6). Xenografted tumor weight was measured 4 weeks later; (E) the size and weight and production situation of subcutaneous tumor were measured; (F) the representative image of tumor metastasis images of nude mice was injected with miR-552-3p sponge (Lv-miR-552-3p sponge) or sponge NC (Lv-miR-NC) cells. The red arrow indicates a metastatic gallbladder carcinoma in the lung. Compared with the control group, *, P<0.05; **, P<0.01; ***, P<0.001. GBC, gallbladder cancer; CCK-8, Cell Counting Kit-8.