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. 2021 Sep;9(17):1374. doi: 10.21037/atm-21-2013

Figure 4.

Figure 4

RGMA is direct target of miR-552-3p in GBC. (A) Prediction of miRNA target genes based on TargetScan database and RNA22 tool; (B) luciferase reporter assay was performed in 293T cells co-transfected with miR-552-3p mimics and pMIR-REPORT luciferase-RGMA 3'UTR (WT) or pMIR-REPORT luciferase-RGMA 3'UTR (MUT); (C) qRT-PCR analyses of RGMA expression levels in SGC-996 and GBC-SD cells transfected with miR-sponge-NC and miR-552-3p sponge or miR-mimic-NC and miR-552-3p mimic; (D) Western blotting analyses of RGMA expression levels in SGC-996 and GBC-SD cells transfected with miR-sponge-NC and miR-552-3p sponge or miR-mimic-NC and miR-552-3p mimc; (E) real-time PCR analysis of the relative expression of RGMA between nontumor and tumor group; (F) the correlation between miR-552-3p expression levels and RGMA expression levels was determined by linear regression analysis (P=0.019, R=−0.366, Pearson’s correlation coefficient). Compared with the control or NATs group, *, P<0.05; **, P<0.01; ***, P<0.001. GBC, gallbladder cancer; RGMA, repulsive guidance molecule BMP co-receptor a; UTR, untranslated region; Mr, molecular mass.