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. 2021 Sep;9(17):1374. doi: 10.21037/atm-21-2013

Figure 5.

Figure 5

Gain and loss of RGMA function abrogates and enhance the impact of miR-552-3p on GBC cell, respectively. (A) The proliferation of RGMA knockdown and overexpression GBC cells was measured by CCK-8 assay; (B) the proliferation of RGMA knockdown and overexpression GBC cells was performed by colony formation assay (dyeing with crystal violet); (C) the migratory ability of RGMA knockdown and overexpression GBC cells was measured by wound-healing assay (scale bar =500 μm); (D) cell proliferation ability was assayed in GBC cells transfected with miR-552-3p sponge and RGMA siRNA or miR-552-3p mimic and RGMA-overexpression vectors by colony formation assay (dyeing with crystal violet); (E) cell migration and invasion were evaluated in GBC cells transfected with miR-552-3p sponge and RGMA siRNA or miR-552-3p mimic and RGMA overexpression vectors (dyeing with crystal violet; scale bar =100 μm). Compared with the NC group, *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. GBC, gallbladder cancer; RGMA, repulsive guidance molecule BMP co-receptor a; CCK-8, Cell Counting Kit-8.