Figure 6.

MiR-552-3p promotes GBC malignant progression by reactivating Akt/β-catenin pathway and EMT. (A) KEGG and PANTHER enrichment pathway analysis for miR-552-3p’s target gene; (B) protein expression levels of RGMA and the activation vs. inhibition situations of Akt after miR-552-3p knockdown or overexpression in GBC cells; (C) protein expression levels of RGMA and the activation vs. inhibition situations of Akt after miR-552-3p knockdown or overexpression in GBC tissues vs. nontumor tissues; (D) the nuclear translocation of β-catenin was visualized by immunofluorescence assay in GBC cells (scale bar =50 μm); (E) E-cadherin and vimentin protein expression levels in the indicated cells were examined by Western blotting. GBC, gallbladder cancer; RGMA, repulsive guidance molecule BMP co-receptor a; EMT, epithelial-mesenchymal transformation; Mr, molecular mass.