TABLE 1.
Characteristics of plasmids used in this study
| Plasmid | Relevant features | Source or reference |
|---|---|---|
| pRS315-CEG1 | CEN/ARS LEU2 CEG1 | 17 |
| pRS316-CEG1 | CEN/ARS URA3 CEG1 | 17 |
| pRS316-CET1 | CEN/ARS URA3 CET1 | 7 |
| YCplac22-KIN28 | CEN/ARS TRP1 KIN28 | 9 |
| YCplac22-kin28-16 | CEN/ARS TRP1 kin28-16(N123D, P206L, V232A, L293S) | 9 |
| pRS426-KIN28 | 2 μm URA3 KIN28 | This study |
| pRS314-hakin28(T17D) | CEN/ARS TRP1 kin28(T17D), C-terminal HAa tag | This study |
| pRS314-hakin28(K36A) | CEN/ARS TRP1 kin28(K36A), C-terminal HA tag | This study |
| pRS314-hakin28(T162A) | CEN/ARS TRP1 kin28(T162A), C-terminal HA tag | This study |
| pDJ29 | srb10Δ::HIS3 | 35 |
| RY2973 | CEN/ARS URA3 SRB10 | 35 |
| pSZH | ctk1Δ::HIS3 | 52 |
| pJYC1513 | CEN/ARS URA3 CTK1 | 33 |
| pRP1-101 | CEN/ARS LEU2 rpb1Δ101 (11 wild-type heptapeptide repeats) | 44 |
| pRP112 | CEN/ARS URA3 RPB1 | 44 |
| pRP114 | CEN/ARS LEU2 RPB1 | 44 |
| pY1WT(10) | CEN/ARS LEU2 rpb1 (10 wild-type repeats) C-terminal HA tag | 55 |
| pY1A2(8)WT(7) | CEN/ARS LEU2 rpb1 (8 S2A, 7 wild-type repeats) C-terminal HA tag | 55 |
| pY1A5(5)WT(7) | CEN/ARS LEU2 rpb1 (5 S5A, 7 wild-type repeats) C-terminal HA tag | 55 |
| rpb1-15 | CEN/ARS LEU2 rpb1-15(T4292A) | 49, 50 |
| rpb1-18 | CEN/ARS LEU2 rpb1-18(G808A) | 49, 50 |
| rpb1-19 | CEN/ARS LEU2 rpb1-19(G4031A) | 49, 50 |
a
HA, hemagglutinin.