TABLE 3.
Summary of genetic analyses between CTD kinase mutants and ceg1-250
| CTD kinase genotypea | Result with growth at 30°C for 3 daysb
|
|
|---|---|---|
| CEG1 | ceg1-250 | |
| KIN28 | ++++ | ++++ |
| kin28(T17D) | ++ | − |
| kin28(K36A) | ++ | ++/+ |
| kin28-16 | ++ | + |
| SRB10 | ++++ | ++++ |
| srb10Δ | ++ | ++ |
| CTK1 | ++++ | ++++ |
| ctk1Δ | ++ | ++ |
a
Construction and analyses of double mutant combinations are described in Materials and Methods.
b
++++, wild-type growth after 3 days; ++, 50% reduced colony size; +, 75% reduced colony size; −, no apparent colonies.