Figure 3.

Efficacy of panitumumab-IR700 NIR-PIT (Pan-PIT) in A431 GFP-luc cells with reducing agents with NaN₃ added in vitro. Membrane damage of A431 GFP-luc cells induced by Pan-PIT with l-NaAA (a) or l-cysteine (b) was measured using PI staining (n = 4, mean ± SEM, versus NIR-PIT without l-NaAA or l-cysteine; one-way ANOVA, followed by Dunnett’s test; **p < 0.01; N.S., not significant). (c) Membrane damage of A431 GFP-luc cells by NIR-PIT with both l-NaAA and l-cysteine was measured using PI staining (n = 4, mean ± SEM, versus NIR-PIT without reducing agents; one-way ANOVA, followed by Dunnett’s test; **p < 0.01; ***p < 0.001; N.S., not significant). Under 10 mM NaN₃ conditions, membrane damage of A431 GFP-luc cells induced by Pan-PIT with l-NaAA (d) or l-cysteine (e) was measured by PI staining (n = 4, mean ± SEM; one-way ANOVA, followed by Tukey’s test; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; N.S., not significant).