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. 2021 Sep 7;4(5):1598–1613. doi: 10.1021/acsptsci.1c00151

Figure 4.

Figure 4

The 1,2,4-triazine ligands are not effective antagonists of mouse GPR84. Ability of increasing concentrations of compound 837 (a) and compound 020 (b) to antagonize effects of EC80 concentrations of 2-HTP at human (circles) and mouse (squares) GPR84 in [35S]GTPγS binding assays performed on membranes of Flp-In TREx 293 cells induced to stably express either a human or mouse GPR84-Gαi2 fusion protein is shown. The low affinity of 837 (c) and compound 020 (d) at mouse GPR84 was confirmed by their lack of ability to substantially effect the observed EC50 of 2-HTP at the mouse GPR84-Gαi2 fusion protein.