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. 2021 Sep 7;4(5):1598–1613. doi: 10.1021/acsptsci.1c00151

Figure 9.

Figure 9

Human-derived THP-1 cells but not mouse-derived RAW-264.7 cells display responses to 2-HTP that are blocked by compound 837. Membranes were prepared from LPS-treated THP-1 cells and RAW-264.7 cells. In both cases, 2-HTPstimulated binding of [35S]GTPγS in a concentration-dependent manner (a, b). Only, however, in THP-1 cell membranes was this blocked by coaddition of compound 837. GLPG1205 was, however, able to block this effect of 2-HTPinRAW-264.7 cells (b). In THP-1 membranes, specific binding of [3H]140 was observed with Kd estimated as 1.3 ± 0.2 nM and Bmax = 866 fmol/mg membrane protein (c).