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. 2021 Jul 19;4(5):1476–1482. doi: 10.1021/acsptsci.1c00134

Figure 2.

Figure 2

Structural and biosafety analysis of rtHLF4. (A) Percentage of ferric ion saturation of lactoferrin and rtHLF4 (1, commercial lactoferrin; 2, flHLF; 3, rtHLF4; each group performed in triplicate). (B) Color of recombinant lactoferrin (left) and rtHLF4 (right; samples prepared at 0.1 mM). (C) Hemolytic assay of purified lactoferrin (maroon) and rtHLF4 (orange) on human red blood cells (inset: hemolytic results using the corresponding rtHLF4 concentrations; n = 10, positive control, 0.1 mg/mL indomethacin). (D) Size exclusion chromatographic separation of flHLF (protein weight, 80 kDa) (arrows indicate dimeric conformation of the isolated protein). (E) Size exclusion separation of rtHLF4 (protein weight, 51 kDa; arrows indicate dimeric and monomeric conformation of the isolated protein).