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. 2021 Sep;9(18):1460. doi: 10.21037/atm-21-4164

Figure 4.

Figure 4

Wog inactivated TRAF6/NF-κB/p65 pathway in PC cells. (A) Bioinformatics analysis was conducted to identify the factors influencing PC progression. The result of KEGG-UP was shown. (B,C) Following 0, 25, 50, or 100 µM Wog stimulation, the concentrations of IL-6, TNF-α, and IL-1β levels in the culture supernatant of PANC-1 and SW1990 cells were measured via ELISA. (D) Following 0, 25, 50, or 100 µM Wog stimulation, the protein levels of TRAF6, TAK1, p-IKKβ/IKKβ, p-P65/P65, and VCAM1 in PANC-1 and SW1990 cells were detected via western blotting. (E) PANC-1 cells were treated with 20 ng/mL IL-1β (TRAF6 activator) with or without 100 µM Wog, and the protein levels of TRAF6, TAK1, p-IKKβ/IKKβ, p-P65/P65, and VCAM1 were assayed by western blotting. (F) The levels of PCNA, CD44, and MMP14 were determined in PANC-1 cells under different treatments. *P<0.05 or **P<0.01 vs. 0 µM Wog stimulation group or Control group, ##P<0.01 vs. IL-1β group. TRAF6, tumor necrosis factor receptor-associated factor 6; NF-κB, nuclear factor-kappa B; PC, pancreatic cancer.