Skip to main content
. 2021 Sep;9(18):1460. doi: 10.21037/atm-21-4164

Figure 6.

Figure 6

Wog exerted anti-PC activity via modulating TRAF6. TRAF6 overexpression plasmid was transfected into PANC-1 cells. Following 100 µM Wog stimulation (A and B), the protein levels of TRAF6, TAK1, p-IKKβ/IKKβ, p-P65/P65, VCAM1, CD44, and MMP14 in cells were measured via western blotting. (C) The stem cell-like transition of PANC-1 cells was observed under a microscope (magnification: 100×). (D) Mesenchymal transition of PANC-1 cells was detected under a microscope (magnification: 200×). PC xenograft animal model was established and subjected to appropriate treatment. (E-G) The tumor picture, volume growth curve, and tumor weight were displayed. (H and I) Immunohistochemistry was conducted to detect TRAF6 and VCAM1 expressions in the cytoplasm, CD44 and MMP14 expressions in the cell membrane (magnification: 400×). *P<0.05 or **P<0.01 vs. Control group, #P<0.05 or ##P<0.01 vs. TRAF6 group. TRAF6, tumor necrosis factor receptor-associated factor 6; MMP14, matrix metalloproteinase 14; PC, pancreatic cancer.