Skip to main content
. 2021 Sep;9(18):1397. doi: 10.21037/atm-21-1885

Figure 2.

Figure 2

Functional characterization of R317S variant. (A) Representative whole-cell current traces recorded from Xenopus laevis oocytes injected with the same amount of cRNA encoding Kv3.1 wild-type (WT) or mutant, R317S, or their 1:1 mixture mimicking the heterozygous expression. Currents were recorded during 0.5 s voltage steps ranging from −60 to +60 mV. (B) Current amplitudes assessed at the end of the voltage step to +60 mV and normalized to the mean current amplitude of WT recorded on the same experimental day. WT (n=136), R317S (n=45), ****, P<0.0001, using Mann-Whitney test. (C) Current amplitudes of WT + H2O (n=111) and WT + R317S (n=26) analyzed at the end of the voltage step to +60 mV and normalized to the mean current amplitude of WT + H2O recorded on the same day. ****, P<0.0001, using unpaired t-test. (D) Conductance-voltage relationships for the WT transcripts and R317S variant expressed alone or together with the WT. V0.5 values in mV: WT 22.6±1.9 (n=36), R317S 16.1±2.6 (n=17) and R317S + WT 21.7±2.9 (n=17) were not significantly different (one-way ANOVA with Dunnett’s multiple comparisons test).