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. 2021 Sep;9(18):1454. doi: 10.21037/atm-21-4249

Figure 5.

Figure 5

ACSL4 regulated the AMPK/mTOR pathway in sev-stimulated SH-SY5Y cells. SH-SY5Y cells were transfected with si-ACSL4 or si-NC for 24 h and treated with 4.1% sev for 6 h, and then tested for the expression of AMPK, pAMPK, mTOR, and p-mTOR using western blot. (A) Representative images for western blot of AMPK, pAMPK, mTOR, and p-mTOR expression in SH-SY5Y cells are shown. Quantification of (B) p-AMPK/AMPK and (C) p-mTOR/mTOR ratios in SH-SY5Y cells. **, P<0.01; ***, P<0.001. ACSL4, acyl-CoA synthetase long chain family member 4; AMPK, 5' AMP-activated protein kinase; mTOR, mammalian target of rapamycin; sev, sevoflurane; pAMPK, phosphorylated AMPK; p-mTOR, phosphorylated mTOR; sev, sevoflurane.