Skip to main content
. 2021 Sep;9(18):1454. doi: 10.21037/atm-21-4249

Figure 6.

Figure 6

ACSL4 knockdown attenuated sev-induced ferroptosis SH-SY5Y cells through the AMPK/mTOR pathway. SH-SY5Y cells were transfected with si-ACSL4 for 12 h, treated with compound C for 12 h and then incubated in the medium plus 4.1% sev for 6 h. (A) Cell viability was analyzed using the CCK-8 assay. Commercial kits were utilized to detect the levels of iron (B), MDA (C), ROS (D,E; DCFDA staining assay), 4-HNE (F), and GSH (G). (H) Western blot analysis of GPX4 protein expression in SH-SY5Y cells. Scale bar =50 μm. *, P<0.05; **, P<0.01; ***, P<0.001. ACSL4, acyl-CoA synthetase long chain family member 4; sev, sevoflurane; AMPK, 5' AMP-activated protein kinase; mTOR, mammalian target of rapamycin; CCK-8, Cell Counting Kit-8; MDA, malondialdehyde; ROS, reactive oxygen species; 4-HNE, 4-hydroxynonenal; GSH, glutathione.