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. 2021 Sep;9(18):1433. doi: 10.21037/atm-21-3903

Figure 3.

Figure 3

Overexpression of miR-146a-5p reduced LPS-induced pro-inflammatory cytokine expression and secretion. (A) The HUVECs transfected with a miR-146a-5 mimic for 48 h; the expression level of miR-146a-5p was detected by qPCR, (B) after the overexpression of miR-146a-5p, the HUVECs were treated with 100 ng/mL LPS for 24 h, and qPCRs were then used to detect the mRNA expression levels of pro-inflammatory cytokine, and (C) the secretion level of IL-6 from HUVECs was measured via ELISA. *, P<0.05; **, P<0.01; ***, P<0.001. LPS, lipopolysaccharide; HUVECs, human umbilical vein endothelial cells; qPCR, quantitative real-time polymerase chain reaction; IL-6, interleukin-6; ELISA, enzyme-linked immunoassay; NC, negative control.